|
KMID : 0380420180420040171
|
|
Journal of Prventive Veterinary Medicine
2018 Volume.42 No. 4 p.171 ~ p.176
|
|
|
Determination of florfenicol residues in swine tissues using high-performance liquid chromatography with ultraviolet photometric detector
|
|
Chae Won-Seok
Yoo Chang-Yeul
Tutkun Lutfiye
Kim Suk
Lee Hu-Jang
|
|
|
Abstract
|
|
|
|
A study of the tissue depletion of florfenicol (FFC) administered orally to pigs at a dose of 0.05 kg/ton feed for 7 days was performed. Sixteen healthy cross swine were administered with FFC. Four treated animals were arbitrarily selected to be sacrificed 1, 3 and 5 days after the end of treatment. FFC residue concentrations in muscle, liver, kidney, and fat were determined using high-performance liquid chromatography (HPLC) with ultraviolet photometric detector at 230 nm. The correlation coefficient (R2) of the calibration curve for florfenicol amine (FFCa) was > 0.997 and the limits of detection and quantification were 0.012 and 0.040 μg/mL, respectively. Recovery rates in swine edible tissues ranged from 79.1 to 93.5%. In the FFC-treated group, FFC residues at 3 days post-treatment were below the maximum residue limits (MRLs) in muscle, kidney and fat, and those at 5 days post-administration were below the MRLs in all edible tissues. These results suggest that the withdrawal period of FFC after the drug treatment might be 5 days, which is a sufficient amount of time for reduction of the FFC residues below the MRLs in all edible tissues.
|
|
|
KEYWORD
|
|
|
HPLC-UV, florfenicol, florfenicol amine, swine edible tissues, withdrawal period
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|